——Precision Concentration, Maximized Sample Integrity——
Centrifugal Vacuum Concentrator plays a crucial role in metabolomics, mainly used for solvent removal and sample concentration during the sample pre-treatment stage. Its efficient and mild characteristics are particularly suitable for handling metabolites that are heat unstable or prone to oxidation.
1. Solvent removal and concentration: Metabolomics samples (such as urine, blood, tissue extracts) often need to be analyzed through liquid chromatography (LC) or gas chromatography (GC). During the pre-treatment stage, the extraction solvents (such as methanol, acetonitrile, water, etc.) need to be removed, and the target metabolites need to be concentrated. Centrifugal Vacuum Concentrator uses low-temperature vacuum evaporation to avoid the destruction of heat-sensitive metabolites (such as vitamins, polyphenols, lipids, etc.) by high temperatures, thereby improving detection sensitivity.
2. Reconstitution of freeze-dried samples: Metabolite samples after freeze-drying need to be re-solubilized with a specific solvent. Centrifugal concentration can precisely control the final volume, ensuring the consistency of concentration for subsequent analyses (such as NMR or MS).
——Outstanding performance, Specially designed for demanding laboratories——
1. Efficient and gentle evaporation: Simultaneously achieve vacuum depressurization and low-temperature centrifugation (usually combined with cooling to -50℃ to -80℃), lowering the boiling point of solvents, rapidly evaporating organic solvents (such as methanol, acetonitrile), retaining the activity of target metabolites, and avoiding degradation of metabolites due to high temperature (such as oxidation or hydrolysis)
2. Reducing oxidation risk: The sealed vacuum environment effectively reduces the oxidation loss of oxygen-sensitive metabolites (such as mercaptans, polyunsaturated fatty acids), ensuring the reliability of the data.
3. High-throughput compatibility: The multi-channel design can handle multiple samples simultaneously, ensuring the sensitivity of trace metabolite detection. Suitable for large-scale metabolomics research (such as clinical cohort analysis).
——Innovative technology, Faster, More accurate and More reliable ——
1. Small volume, Big capacity: Concentration of 180-264 pcs 1.5/2.0ml centrifuge tubes in a single run.
2. Vacuum control system：Made in Germany，Stable, Accurate, Efficient.
3. New electromagnetic drive system：High torque, Low vibration,Corrosion resistance.
4. 2-stage temperature control,protecting sample safety effectively.
5. 4 groups program shortcut key, 30 groups user-defined programs.
6. Imported pressure relief valve with automatic vacuum release, safe and easy to operate.
——Versatile Applications,Empowering research in multiple fields——
Applicable to proteomics, genomics, molecular biology, natural product research.
1. Molecular Biology & Genetic Research: DNA/RNA concentration, protein purification, nucleotide/primer processing.
2. Pharmaceutical & Drug Development: Drug screening, natural product extraction, metabolomics research.
3. Food Safety & Environmental Testing: Pesticide residue analysis, veterinary/antibiotic testing, environmental pollutant monitoring.
4. Chemical Synthesis & Materials Science: Purification of organic synthesis products, preparation of nanomaterials, polymer research.
5. Clinical Diagnosis & Medical Research: Pre-treatment of body fluid samples (serum, urine), sample preparation for mass spectrometry (MS), virus/vaccine research.
——Centrifugal concentration workflow and precautions——
1. Sample extraction: Metabolites are extracted from biological samples using a mixture of methanol, acetonitrile and water.
2. Centrifugal concentration: Organic solvents are removed in a Centrifugal Vacuum Concentrator, and the remaining aqueous phase can be further processed by freeze-drying.
3. Derivatization (if necessary): Before GC-MS analysis, the concentrated sample may need to be derivatized (such as silylation), and then further concentrated to remove excess reagents.
4. Instrumental analysis: The concentrated metabolites are dissolved in a suitable solvent for LC-MS or GC-MS analysis and then analyzed.
The following are the matters that need attention during the concentration process:
→ Loss of volatile metabolites: Low boiling point compounds such as short-chain fatty acids and aldehydes may volatilize during the concentration process. The vacuum level or nitrogen blow concentration method should be optimized.
→ Cross-contamination: During high-throughput operation, ensure the rotor is clean to avoid residual samples between samples.
→ Solvent selection: High boiling point solvents (such as DMSO) may be difficult to completely remove. The selection should be optimized in combination with subsequent steps.
